Related Experiment Video

Updated: Jun 21, 2025

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

9.4K

An Analytical Protocol for Detecting Antibody Titer Levels in Serum/Saliva by Indirect Enzyme-Linked Immunosorbent

Jingwen Wang1, Sahra Bashiri1, Istvan Toth2,3,4

  • 1The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|July 12, 2024
PubMed

Keywords:
Antibody titersAntigensELISAEnzyme-linked immunosorbent assaySemiquantitative analysis

More Related Videos

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens09:08

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Published on: September 12, 2016

8.7K
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay08:11

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

Published on: September 6, 2016

18.7K

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
12.7K