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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

355
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
355
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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Related Experiment Video

Updated: Jun 21, 2025

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Label-Free Assessment of Neuronal Activity Using Raman Micro-Spectroscopy.

Yuka Akagi1, Aya Norimoto1, Teruhisa Kawamura2

  • 1Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba 305-8565, Ibaraki, Japan.

Molecules (Basel, Switzerland)
|July 13, 2024
PubMed
Summary

The Paint Raman Express Spectroscopy System (PRESS) effectively measures neuronal activity and chemical responses in individual neurons and ganglia. This label-free technology aids in understanding neuronal population dynamics and pharmacological responses for drug discovery.

Keywords:
Raman spectroscopyinduced pluripotent stem cellsneuronal activitynon-label evaluation

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Area of Science:

  • Neuroscience
  • Spectroscopy
  • Machine Learning

Background:

  • Assessing neuronal population output is vital for understanding nervous system functions.
  • Prior work developed rapid Raman spectroscopy and machine learning for label-free cell state evaluation.

Purpose of the Study:

  • To investigate the capability of the Paint Raman Express Spectroscopy System (PRESS) in assessing neuronal activities.
  • To examine chemical responses of human-induced pluripotent stem cell-derived neurons and ganglia.

Main Methods:

  • Utilized the PRESS for rapid acquisition of Raman spectra from individual neurons and autonomic neuron ganglia.
  • Employed machine learning, principal component analysis (PCA), and partial least squares discriminant analysis (PLSDA) for data analysis.
  • Tested ligand responsiveness using glutamate and nicotine in a calcium-dependent manner.

Main Results:

  • PRESS acquired high signal-to-noise Raman spectra from neurons and ganglia within seconds.
  • Detected calcium-dependent neuronal activity in response to glutamate and nicotine.
  • Observed dose-dependent changes in neuronal activity, demonstrating PRESS's sensitivity.

Conclusions:

  • The PRESS system can effectively assess individual neuronal activity and neuronal population dynamics.
  • Demonstrated the system's utility in evaluating pharmacological responses and ligand responsiveness.
  • Highlights PRESS as a promising tool for drug discovery and regenerative medicine research.