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Related Experiment Video

Updated: Jun 21, 2025

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
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Pilot Study for Isolation of Stromal Vascular Fraction with Collagenase Using an Automated Processing System.

Gershon Zinger1, Yoav Gronovich2, Adi Maisel Lotan2

  • 1Department of Orthopedic Surgery, Hand Unit, The Eisenberg R&D Authority, Shaare Zedek Medical Center, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9103102, Israel.

International Journal of Molecular Sciences
|July 13, 2024
PubMed
Summary

The Automated Cell Station (ACS) efficiently isolates sterile adipose-derived stromal cells from adipose tissue. This system provides a valuable source of stromal vascular fraction (SVF) for therapeutic applications.

Keywords:
ACSASCsSVFadipose tissuecell countregenerative cellsstromal vascular fraction

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Autologous adipose-derived stromal cells (ASCs) have significant therapeutic potential.
  • These cells are part of the stromal vascular fraction (SVF) isolated from adipose tissue.
  • Closed automated systems offer a method for cell isolation from adipose tissue.

Purpose of the Study:

  • To evaluate the Automated Cell Station (ACS) for yield, purity, and cellular characterization of SVF.
  • To assess the stemness criteria and sterility of the isolated SVF.
  • To determine the efficiency of the ACS system for processing adipose tissue for cell therapy.

Main Methods:

  • SVF was isolated from three donors using the Automated Cell Station (ACS).
  • Characterization included cell yield, viability, immunophenotyping (flow cytometry), differentiation potential, and colony-forming units.
  • Sterility was assessed by testing for endotoxin and collagenase residuals.

Main Results:

  • The ACS system yielded an average of 7.9 mL SVF with 19 × 10^6 nucleated cells at 85% viability.
  • Immunophenotyping revealed a heterogeneous cell population including ASCs (23%), macrophages (24%), and endothelial cells (5%).
  • The SVF demonstrated multipotent differentiation capabilities (adipogenic, chondrogenic, osteogenic) and was free of endotoxin and collagenase residuals.

Conclusions:

  • The ACS system efficiently processes adipose tissue within a single surgical procedure.
  • The system yields a sterile, concentrated SVF, rich in ASCs, suitable for therapeutic use.
  • This automated approach provides a reliable source of cells for regenerative medicine applications.