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Related Concept Videos

Insulin Formulations: Types and Delivery01:27

Insulin Formulations: Types and Delivery

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Insulin preparations are categorized by their duration of action into short-acting and long-acting types. Two strategies are used to modify insulin's absorption and pharmacokinetic profile: slowing the absorption post-subcutaneous injection, or altering human insulin's amino acid sequence or protein structure. These changes retain the insulin's ability to bind to the insulin receptor, but alter its behavior in solution or after injection.
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Patch Clamp01:18

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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An Insulin-Modified pH-Responsive Nanopipette Based on Ion Current Rectification.

Xu-Fan Wang1,2,3, Yi-Fan Duan1,2, Yue-Qian Zhu1,2,3

  • 1Department of Histology and Embryology, School of Basic Medical Sciences, Xuzhou Medical University, Xuzhou 221004, China.

Sensors (Basel, Switzerland)
|July 13, 2024
PubMed
Summary

Insulin modification significantly enhances nanopipette pH sensing performance. These modified nanopipettes show improved sensitivity and selectivity for practical pH detection applications.

Keywords:
bovine insulinion current rectificationnanopipettespH responsesilanization and amidation

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Area of Science:

  • Nanotechnology
  • Biosensing
  • Analytical Chemistry

Background:

  • Nanopipette properties are determined by inner wall materials, impacting sensing capabilities.
  • Improving sensitivity and selectivity of nanopipettes for pH sensing is a key challenge.
  • Current pH sensing nanopipettes face limitations in practical applications.

Purpose of the Study:

  • To develop insulin-modified nanopipettes for enhanced pH sensing.
  • To investigate the mechanism and optimization of insulin modification for nanopipettes.
  • To evaluate the performance of insulin-modified nanopipettes in terms of sensitivity, selectivity, and reversibility.

Main Methods:

  • Two-step modification of nanopipette inner walls involving silanization and amidation.
  • Introduction of insulin onto the inner walls of nanopipettes.
  • Measurement of ion current rectification for pH response analysis.
  • Investigation of solution pH and insulin concentration effects on modification.

Main Results:

  • Insulin-modified nanopipettes exhibited a 4.29-fold enhancement in pH response intensity compared to bare nanopipettes.
  • A linear pH response was observed in the range of 2.50 to 7.80.
  • The modified nanopipettes demonstrated good reversibility and selectivity for pH sensing.
  • Optimal insulin introduction conditions were identified through systematic investigation.

Conclusions:

  • Insulin modification is an effective strategy to significantly improve nanopipette pH sensing performance.
  • The developed insulin-modified nanopipettes offer enhanced sensitivity and selectivity for practical pH detection.
  • This approach contributes to the advancement of pH-responsive nanopipette technology and applications.