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Common bean polyphenolic enriched extracts decrease reactive oxygen species induced by heavy metals and polycyclic

David Fonseca Hernandez1, Luis Mojica1, Eugenia Lugo Cervantes1

  • 1Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Guadalajara 44270, Mexico.

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|July 13, 2024
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Summary

Common bean extracts rich in phenolic compounds show potent antioxidant effects against air pollution-induced skin damage. These natural compounds protect human skin cells from oxidative stress caused by particulate matter (PM10).

Keywords:
AMPKAnthocyaninsInflammationParticulate matterPolyphenolsSirt1

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Area of Science:

  • Dermatology and Environmental Health
  • Nutraceuticals and Phytochemistry

Background:

  • Industrialization and urban sprawl increase coarse particulate matter (PM10), a major air pollutant.
  • PM10 exposure poses a significant threat to human skin health, accelerating aging and causing oxidative stress.
  • Phenolic compounds, abundant in legumes like common beans, are known for their antioxidant properties.

Purpose of the Study:

  • To evaluate the antioxidant efficacy of phenolic-enriched extracts (PHE) from black bean (BB) and pinto bean (PB) varieties.
  • To assess the protective effects of PHE and pure phenolic compounds against PM10-induced oxidative stress in human dermal fibroblasts.

Main Methods:

  • Phenolic-enriched extracts (PHE) from Phaseolus vulgaris L. (BB and PB) were prepared.
  • Quantification of key phenolic compounds (anthocyanins, phenolic acids) and pure compounds (rutin, catechin, gallic acid) was performed.
  • Human dermal fibroblast cell lines (Hs27 and Hs68) were exposed to PM10 (100 μg/mL) to induce oxidative stress.
  • The antioxidant activity of PHE and pure compounds was measured, including binding affinity to AMPK and Sirt1.

Main Results:

  • Petunidin-3-O-glucoside and gallic acid were identified as major compounds in PHE-BB.
  • PHE-BB and PHE-PB significantly reduced PM10-induced oxidative stress in fibroblasts by 69% and 80%, respectively.
  • Delphinidin-3-O-glucoside and quercetin-3-D-galactoside exhibited high binding affinity to AMPK and Sirt1.
  • Rutin supplementation increased Sirt1 expression by 30% in PM10-treated Hs27 cells.

Conclusions:

  • Common bean extracts possess significant antioxidant potential against PM10-induced oxidative stress in skin cells.
  • Phenolic compounds in common beans may offer a natural strategy to mitigate skin aging and damage caused by air pollution.
  • Further research into these plant-derived compounds could lead to novel skincare formulations for environmental protection.