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Updated: Jun 21, 2025
Generation of Mice Derived from Induced Pluripotent Stem Cells
Published on: November 29, 2012
Generation of a homozygous (MCRIi031-A-3) WT1 knockout human iPSC line
Svenja Pachernegg1, Gorjana Robevska2, Lucas G A Ferreira3
1Reproductive Development, Murdoch Children's Research Institute, Melbourne, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
Abstract:
The transcription factor WT1 plays a critical role in several embryonic developmental processes such as gonadogenesis, nephrogenesis, and cardiac development. We generated a homozygous (MCRIi031-A-3) WT1 knockout induced pluripotent stem cell (iPSC) line from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. The cells exhibit a normal karyotype and morphology, express pluripotency markers, and have the capacity to differentiate into the three embryonic germ layers. These cell lines will allow us to further explore the role of WT1 in critical developmental processes.
