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Recombinant Biogenesis and Analysis of Cytochrome c Species
Alicia N Kreiman1, Tania Yeasmin2, Molly C Sutherland3
1Department of Biological Sciences, University of Delaware, Newark, DE, USA.
Abstract:
Recombinant expression and biogenesis of cytochrome c species is a simple and efficient method for the production of holocytochrome c species, thus presenting an avenue for the study of cytochrome c or the cytochrome c biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant E. coli production of holocytochrome c utilizing the System I (CcmABCDEFGH) bacterial cytochrome c biogenesis pathway, followed by analysis of cytochrome c species by cell lysis and heme stain.
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