Related Experiment Video
Updated: Jun 21, 2025

09:43
Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
12.1K
Radiolabeling Heme and Porphyrin with 14C-Glycine or 14C δ-Aminolevulinic Acid
Diane M Ward1, Samantha Gillis2,3, Yvette Y Yien4
1Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 15, 2024
Abstract:
Radiolabeling enables the quantitation of newly synthesized heme and porphyrin, allowing us to distinguish heme synthesis rates from total cellular heme. Here, we describe a protocol for labeling heme with 14C-glycine or ALA and the sequential extraction of heme and porphyrin from the same samples for quantitation by liquid scintillation.

