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Substantia Innominata Glutamatergic Neurons Modulate Sevoflurane Anesthesia in Male Mice
Li Yang1, Fang Fang1, Wen-Xu Wang2
1From the Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, China.
Anesthesia and Analgesia
|July 15, 2024
Summary
Glutamatergic neurons in the substantia innominata (SI) help control how quickly animals wake up from sevoflurane anesthesia. Activating these neurons speeds emergence from general anesthesia (GA).
Area of Science:
- Neuroscience
- Anesthesiology
- Sleep research
Background:
- Brain regions promoting wakefulness also aid emergence from general anesthesia (GA).
- Glutamatergic neurons in the substantia innominata (SI) are involved in arousal and motivation.
- The role of SI glutamatergic neurons in sevoflurane anesthesia is unexplored.
Purpose of the Study:
- To investigate the role of SI glutamatergic neurons in regulating sevoflurane anesthesia.
- To examine the involvement of the SI-LH glutamatergic pathway in consciousness regulation during GA.
Main Methods:
- Fiber photometry, chemogenetics, and optogenetics in adult male mice.
- Behavioral tests and cortical electroencephalogram recordings.
- Investigated SI glutamatergic neuron activity and projections to the lateral hypothalamus (LH).
Main Results:
- SI glutamatergic neuron activity decreased during sevoflurane-induced loss of consciousness (LOC).
- Activation of SI glutamatergic neurons prolonged anesthesia induction and shortened emergence.
- Inhibition of these neurons facilitated sevoflurane anesthesia; optogenetic activation induced emergence.
Conclusions:
- SI glutamatergic neuronal activity facilitates emergence from sevoflurane anesthesia.
- The SI-LH glutamatergic pathway regulates consciousness during general anesthesia.
