GC/MS Analysis and Compounds Isolation of Lycium shawii Petroleum Ether Seeds Extract for Regulating Nrf2/OH-1...

Acrylamide is formed during heating of starchy foods at high temperature and induces reproductive toxicity. Our study is designed to evaluate the chemical constitution and anti-infertility effect of Lycium shawii seeds extract on female rats. Nutritional profile was estimated, and major active compounds were isolated and identified. Biological evaluation of L. shawii extract on female rats was performed and measured by prolactin, follicular stimulating hormone, luteinizing hormone, estradiol, progesterone, tumor necrosis factor-α, interleukin-6, heme oxygenase-1, nuclear respiratory factor-2, malondialdehyde, glutathione, DNA fragmentation, and ovarian architecture parameters. Data revealed the presence of ɤ-tocopherol, vitamin C, magnesium, and 38 bioactive compounds in the fractions of L. shawii. Major constituents from gas chromatography/mass spectrometry (GC/MS) were 9, 12-octadecadienoic acid (Z, Z), methyl ester, 2,7-octadiene-1,6-diol, and 2,6-dimethyl hydroxy linalool but further five compounds (i.e., lupenone, betulin, lupeol acetate, stigmasterol, and β-sitosterol-d-glucoside) were isolated and identified. Treatment of rats with the seeds extract post acrylamide administration ameliorated female sex hormones, oxidative stress, inflammation, DNA damage, and ovarian structure. In conclusion, L. shawii petroleum ether seeds fraction may be considered a nutraceutical agent for improving infertility disorders, oxidative stress, and inflammation due to its richness with biologically active phenolic and flavonoids compounds.