An improved method for the concentration of cerebrospinal fluid cells by suction tip and sedimentation chamber

To concentrate cells from cerebrospinal fluid, a new method was developed. The technique is a modification of the cell sedimentation method of Sayk. The fluid is absorbed after passing through a Nuclepore filter, pore size 0.4 micron. With the aid of simple utensils it is possible to obtain a cell preparation of high quality using only 0.3 ml of cerebrospinal fluid. The cytomorphology is as good as that achieved by Sayk's sedimentation technique. The cell yield was determined using an accurate and precise method to estimate the number of cells present in the cerebrospinal fluid. The average cell yield is 90%, and all staining methods are applicable. The system is easily modified, and can be suited to many other fluids and purposes.