Related Experiment Video
Updated: Jun 21, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
A (RP)UHPLC/UV analytical method to quantify dsRNA during the mRNA vaccine manufacturing process
Sara Sousa Rosa1,2,3, Shuran Zhang1, Yustika Sari1
1Department of Biochemical Engineering, University College London, Gordon Street, London, WC1E 6BT, UK. marco.marques@ucl.ac.uk.
None:
dsRNA is a product related impurity produced during the mRNA manufacturing process. The established immuno-based detection methods lack the flexibility and speed required to be applied throughout the manufacturing process. The RP-HPLC method developed outperforms these in terms of precision, broader detection range, LOD and LOQ, as well as in output variance. Using this method, dsRNA can be quantified in under 30 min for a single sample.

