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[Culturing of chlorine-damaged E. coli bacteria using the membrane filteration technic]
Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale B, Hygiene
|June 1, 1985
Summary
Membrane filtration with Endo-agar significantly reduces recoverable chlorine-impaired E. coli, making it less effective than liquid enrichment. Endo-agar
Area of Science:
- Environmental microbiology
- Water quality testing
- Bacteriology
Background:
- Standard methods for detecting E. coli in water are crucial for public health.
- Membrane filtration and liquid enrichment are common techniques for bacterial enumeration.
- Previous studies have highlighted challenges in standardizing membrane filtration methods.
Purpose of the Study:
- To evaluate the efficacy of the membrane-filtration-Endo-agar method for detecting chlorine-impaired E. coli.
- To compare the performance of membrane filtration with liquid enrichment techniques.
- To identify limitations of the Endo-agar medium in bacterial recovery.
Main Methods:
- Comparison of membrane filtration using Endo-agar with liquid enrichment methods.
- Assessment of E. coli recovery rates under chlorine stress.
- Evaluation of different broth formulations, including lactose-peptone (L.P.)-broth and casein-soya (C.S.)-broth.
Main Results:
- The membrane-filtration-Endo-agar method significantly reduced the recovery of chlorine-impaired E. coli compared to liquid enrichment.
- Toxicity of the Endo-agar medium, rather than filter suitability, was identified as a primary limitation.
- Lactose-peptone (L.P.)-broth yielded unsatisfactory results when compared to casein-soya (C.S.)-broth.
Conclusions:
- The membrane-filtration-Endo-agar method is not equivalent to liquid enrichment for detecting chlorine-impaired E. coli.
- The toxicity of Endo-agar poses a significant challenge for accurate bacterial enumeration.
- Casein-soya (C.S.)-broth shows superior performance over lactose-peptone (L.P.)-broth in this context.