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Whole Blood Aggregometry in Mice.

Siobhan Branfield¹, Yashieta Somani^1,2, A Valance Washington¹

¹Department of Biological Sciences, Oakland University, Rochester Hills, Michigan.

Current Protocols

|July 17, 2024

Summary

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This study presents a simple protocol for murine whole blood aggregometry, a valuable tool for hematology research. Standardizing this method enhances experimental reliability and aids in understanding platelet function in mice.

Area of Science:

Hematology
Platelet Physiology
Translational Research

Background:

Aggregometry is vital for studying platelet function in hematology research and clinical diagnostics.
Existing methods like Light Transmission Aggregometry (LTA) and whole blood aggregometry have distinct advantages.
While human protocols exist, a standardized murine whole blood aggregometry protocol is lacking.

Purpose of the Study:

To introduce a simple, basic protocol for performing whole blood aggregometry in murine models.
To provide researchers with an accessible alternative to Light Transmission Aggregometry (LTA) for platelet function analysis in mice.
To promote standardization of murine whole blood aggregometry for improved experimental reproducibility.

Main Methods:

Development and description of a basic protocol for murine whole blood aggregometry.

Keywords:

aggregometry hematology light transmission aggregometry lumi‐aggregometry murine models phenylhydrazine platelet activation platelet disorders platelet function whole blood aggregometry

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Inclusion of a support protocol for inducing phenylhydrazine-induced anemia in wild-type mice.

Inclusion of a protocol for determining hematocrit percentage in mice.

Main Results:

A straightforward protocol for murine whole blood aggregometry is successfully outlined.
The protocol offers a physiologically relevant approach to platelet function assessment in mice.
The described methods facilitate hematological research in murine models.

Conclusions:

Standardizing whole blood aggregometry protocols in mice is crucial for enhancing experimental reliability.
This protocol serves as a valuable resource for hematology researchers studying platelet function.
Adoption of this method can facilitate translational research by improving consistency in murine models.