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Related Concept Videos

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

977
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
977

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Using Ex Vivo Tonsil Organoids to Study Memory B Cells.

Zachary W Wagoner1, Mahina Tabassum Mitul1, Lisa E Wagar2

  • 1Department of Physiology and Biophysics, Institute for Immunology, Center for Virus Research, and Vaccine R&D Center, University of California Irvine, Irvine, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 17, 2024
PubMed
Summary
This summary is machine-generated.

New tools are needed to study memory B cells (MBCs) and their role in fighting infections. This study introduces a human lymphoid tissue organoid system to analyze MBCs within their natural environment, offering insights into immune responses.

Keywords:
Antigen-specific B cellsHuman immunologyImmune organoidsLymphoid tissueVaccine response

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Area of Science:

  • Immunology
  • Cell Biology
  • Infectious Diseases

Background:

  • Understanding memory B cell (MBC) development and function is crucial for sustained protection against recurrent infections.
  • Current methods for studying human MBCs primarily rely on blood samples, limiting insights into their behavior within lymphoid tissues.
  • Lymphoid tissues are central to orchestrating adaptive immune responses.

Purpose of the Study:

  • To introduce a novel high-throughput organoid system derived from primary human lymphoid tissues.
  • To enable the study of memory B cell dynamics within an entirely human system.
  • To characterize pre-existing antigen-specific MBCs and understand inter-individual variation.

Main Methods:

  • Development of a high-throughput organoid system using primary human lymphoid tissues.
  • Recapitulation of key features of adaptive immune responses within the organoid system.
  • Analysis of memory B cell characteristics and responses to various stimuli.

Main Results:

  • The organoid system successfully recapitulates hallmarks of adaptive immune responses.
  • The system captures significant inter-individual variation in immune responses.
  • Enables characterization of antigen-specific memory B cells in a human context.

Conclusions:

  • Human lymphoid tissue organoids provide a powerful platform for studying memory B cell dynamics.
  • This approach offers valuable insights into the role of MBCs in sustained immunity.
  • Facilitates research into adaptive immunity within a more physiologically relevant system.