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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Updated: Jun 20, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

Measuring B Cell Chromatin Accessibility by One-Step ATAC-seq.

Sakeenah L Hicks¹, Christopher D Scharer²

¹Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 17, 2024

Summary

This summary is machine-generated.

The Assay for Transposase Accessible Chromatin (ATAC)-seq protocol efficiently maps accessible chromatin. This method uses Tn5 transposase for DNA fragmentation and adapter ligation, creating sequencing libraries from minimal cell inputs.

Keywords:

ATAC-seq Chromatin accessibility Epigenetic Gene regulation Sequencing library Tn5 transposase

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Last Updated: Jun 20, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

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Published on: March 22, 2019

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Understanding chromatin accessibility is crucial for studying gene regulation.
Existing methods often require large cell numbers.
A need exists for efficient chromatin mapping techniques.

Purpose of the Study:

To present an optimized Assay for Transposase Accessible Chromatin (ATAC)-seq protocol.
To enable global mapping of accessible chromatin with limited cell inputs.

Main Methods:

Utilizing the Tn5 transposase for simultaneous DNA fragmentation and adapter tagging.
Employing dual indexing primers for PCR amplification.
Generating size-specific sequencing libraries from nuclei transposition.

Main Results:

The protocol is optimized for generating global maps of accessible chromatin.
It effectively works with a range of limited cell inputs.
A streamlined One-Step workflow is outlined.

Conclusions:

The optimized ATAC-seq protocol provides a powerful tool for chromatin accessibility studies.
It allows for high-resolution genomic profiling using minimal biological material.
This method facilitates comprehensive epigenetic analysis.