Structure and expression of murine fourth complement component (C4) and sex-limited protein (Slp)

During the past 4 years we have used recombinant DNA technology to build upon previous genetic and biochemical studies of the C4 and Slp genes and their products. We have isolated DNA probes specific for C4 and Slp, determined the complete sequences of C4 and Slp molecules, established the role of liver mRNA levels in determining serum C4 and Slp levels identified the C4 and Slp genes in Balb/c mice, and begun to probe the structures of the C4 and Slp genes in a variety of inbred mouse strains. This work has provided the tools and a framework for future studies aimed at understanding the multiple functions of the C4 protein and the regulatory mechanisms controlling the expression of the C4 and Slp genes.