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Updated: May 14, 2026

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
Published on: February 13, 2017
Protocol for transducing human primary epithelial prostate cells and patient-derived organoids with high efficiency
Cynthia Jobin1, Mario Harvey2, Aurélie Lacouture1
1Department of Molecular Medicine, Université Laval, Quebec City, QC, Canada; Endocrinology and Nephrology Division, CHU de Québec-Université Laval Research Center (CRCHUQ-UL), Quebec City, QC, Canada; Cancer Research Center (CRC) of Université Laval, Quebec City, QC, Canada.
None:
Patient-derived organoids (PDOs) are now used to study many diseases, including prostate cancer. Here, we present a protocol for the transduction of human epithelial prostate cells and PDOs. We describe the steps for producing lentiviruses and transducing PDOs with high efficiency to obtain either overexpression or knockdown of specific genes. More generally, this protocol represents an efficient lentiviral transduction technique to study cell biology using various organoid models.
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