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Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants
Molecular & General Genetics : MGG
|August 1, 1979
Abstract:
Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.