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Direct Itaconate Production from Brown Macroalgae Using Engineered Vibrio sp. dhg.
Jo Hyun Moon1, Sunghwa Woo1, Hyo Jeong Shin1
1Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea.
Journal of Agricultural and Food Chemistry
|July 20, 2024
Summary
This study demonstrates direct itaconate production from brown macroalgae using engineered Vibrio sp. dhg. The engineered strain achieved significant itaconate titers, highlighting algae as a sustainable feedstock for chemical bioproduction.
Area of Science:
- Biotechnology
- Synthetic Biology
- Sustainable Chemistry
Background:
- Itaconate is a valuable platform chemical, but its production is limited by crop-based feedstock availability.
- Brown macroalgae offer a sustainable, high-yield alternative biomass source for industrial applications.
- Vibrio sp. dhg is an emerging platform for efficient brown macroalgal biorefineries.
Purpose of the Study:
- To demonstrate direct itaconate production from brown macroalgae.
- To engineer Vibrio sp. dhg for enhanced itaconate biosynthesis using macroalgal substrates.
- To evaluate the potential of macroalgae as a feedstock for itaconate bioproduction.
Main Methods:
- Engineered Vibrio sp. dhg strain VIC was developed.
- Heterologous expression of cis-aconitate decarboxylase (Cad) from Aspergillus terreus.
- Deletion of isocitrate dehydrogenase (icd) gene in Vibrio sp. dhg.
Main Results:
- VIC strain achieved itaconate titers of 2.5 g/L from alginate and mannitol mixture.
- VIC strain produced 1.5 g/L itaconate from 40 g/L raw Saccharina japonica.
- Demonstrated feasibility of direct itaconate production from raw macroalgae.
Conclusions:
- Brown macroalgae are a viable and sustainable feedstock for itaconate bioproduction.
- Engineered Vibrio sp. dhg serves as an effective platform for macroalgal biorefining.
- This study advances the development of bio-based itaconate production processes.
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