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Related Experiment Video

Updated: Jun 20, 2025

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
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Fluorescent probe for imaging intercellular tension: molecular force approach.

Xiao-Hong Wang1, Ming Wang2, Jian-Bin Pan3

  • 1School of Chemical and Pharmaceutical Engineering, Hefei Normal University 230061 Hefei Anhui China.

RSC Advances
|July 22, 2024
PubMed
Summary

Researchers developed a new Au-DNA probe to visualize cellular mechanical force. This molecular tension probe enables sensitive imaging of intercellular tension in living cells.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Cellular mechanical force is vital for biological processes like wound healing and metastasis.
  • Visualizing intercellular tension is challenging but crucial for understanding cell behavior.

Purpose of the Study:

  • To design and validate a novel molecular tension probe for imaging cellular mechanical force.
  • To develop a universally applicable method for preparing Au-DNA intercellular tension probes.

Main Methods:

  • Utilized gold nanoparticles conjugated with DNA hairpins as the core of the tension probe.
  • Incorporated a fluorophore-quencher pair within the DNA hairpin structure.
  • Anchored the probe between cells to detect forces that open the hairpin and de-quench fluorescence.

Main Results:

  • Successfully created Au-DNA intercellular tension probes with sensitive visualization capabilities.
  • Demonstrated the probe's effectiveness in imaging cellular force in living cells.
  • The probe allows for sensitive visualization of cellular force through de-quenching of fluorescence.

Conclusions:

  • The developed Au-DNA molecular tension probe offers a simple and efficient method for imaging intercellular tension.
  • This technology has universal applicability for studying cellular forces in various biological contexts.