NIR afterglow nanosystem for photodynamic therapy

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Cancer Research

Background:

• Reactive oxygen species (ROS) are crucial in cancer therapy, inducing immunogenic cell death (ICD).
• Precise control of ROS levels is essential to avoid adverse immune responses and optimize therapeutic outcomes.
• Current methods lack real-time monitoring of ROS production during treatment.

Purpose of the Study:

• To develop an innovative afterglow nanosystem for real-time monitoring of ROS levels.
• To evaluate the efficacy of the engineered nanosystem in photodynamic therapy (PDT).
• To establish a correlation between afterglow luminescence intensity and cancer cell inhibition rate.

Main Methods:

• Engineering of an afterglow nanosystem (Ru/CYQ@CPPO) for ROS detection.
• Comparative analysis of singlet oxygen generation and afterglow luminescence with indocyanine green (ICG).
• In vitro evaluation of Ru/CYQ@CPPO in photodynamic therapy using 450 nm irradiation.
• Correlation analysis between afterglow intensity and cancer cell inhibition.

Main Results:

• Ru/CYQ@CPPO demonstrated significantly enhanced and prolonged afterglow luminescence compared to ICG.
• The nanosystem exhibited superior singlet oxygen generation.
• In vitro studies confirmed remarkable photodynamic therapy efficacy at 450 nm.
• A strong correlation (R² = 0.987) was found between afterglow luminescence intensity and cancer cell inhibition.

Conclusions:

• The developed afterglow nanosystem enables real-time ROS monitoring for precise cancer therapy.
• Ru/CYQ@CPPO offers a promising platform for enhanced photodynamic therapy with improved safety and efficacy.
• This technology provides a quantitative method to assess therapeutic response in real-time.