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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells During Rift Valley Fever Virus

Luke Adam White^1,2, Katherine E Havranek¹, J Stephen Lodmell³

¹Division of Biological Sciences, University of Montana, Missoula, MT, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 22, 2024

Summary

RNA sequencing (RNAseq) reveals host cell gene expression changes during viral infections. This method helps identify immune responses and virus-driven alterations for better understanding of pathogen-host interactions.

Area of Science:

Virology
Molecular Biology
Genomics

Background:

Viral infections induce significant transcriptional alterations in host cells.
These changes include differential gene expression, alternative splicing, and mRNA degradation.
Understanding these changes is crucial for studying host immune responses and viral pathogenesis.

Purpose of the Study:

To outline a protocol for mRNA sequencing (RNAseq) in Rift Valley fever virus-infected cell cultures.
To provide a method for analyzing host cell transcriptional changes during viral infection.
To aid researchers in understanding host-pathogen interactions at the molecular level.

Main Methods:

Infection of cell cultures with Rift Valley fever virus.
Isolation of RNA and subsequent mRNA sequencing (RNAseq).
Bioinformatic analysis of gene expression data.

Main Results:

Identification of differentially expressed genes in response to viral infection.
Characterization of host immune pathways activated during infection.
Detection of virus-mediated alterations in host gene expression.

Conclusions:

RNAseq is an effective tool for studying host responses to viral infections.
The protocol facilitates the analysis of complex gene expression changes.
This approach enhances the understanding of viral pathogenesis and host-virus interactions.

Keywords:

Alternative splicing Gene expression Host pathogen interaction MP-12 RNAseq Rift Valley fever virus Transcriptomics

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