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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Related Experiment Video

Updated: May 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.

Andrea Bucci1,2, Giorgio Tortarolo1,3, Marcus Oliver Held1

  • 1Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.

Nature Communications
|July 23, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces an enhanced confocal microscope for precise single-particle tracking and fluorescence lifetime measurement. The new system improves spatiotemporal resolution for biological research.

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A Protocol for Real-time 3D Single Particle Tracking
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Last Updated: May 8, 2026

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A Protocol for Real-time 3D Single Particle Tracking
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A Protocol for Real-time 3D Single Particle Tracking

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Area of Science:

  • Biophysics
  • Microscopy
  • Cell Biology

Background:

  • Single-particle tracking (SPT) is crucial for understanding biological particle dynamics.
  • Existing SPT methods face trade-offs in resolution, complexity, and data acquisition.
  • There is a need for advanced microscopy techniques with improved spatiotemporal precision.

Purpose of the Study:

  • To enhance a confocal laser scanning microscope for superior single-particle tracking.
  • To integrate fluorescence lifetime measurement with real-time particle localization.
  • To overcome limitations of current SPT techniques for biological investigations.

Main Methods:

  • Augmented a confocal laser scanning microscope with an asynchronous single-photon avalanche diode array detector.
  • Implemented a real-time feedback system to maintain particle centering within the excitation volume.
  • Combined single-particle tracking with fluorescence lifetime measurements using photon detection.

Main Results:

  • Achieved 40 nm lateral and 60 nm axial localization precision with 100 photons.
  • Enabled sub-millisecond temporal sampling for real-time tracking, refining to microsecond scale offline.
  • Successfully tracked fluorescent beads and lysosomes in living cells, measuring membrane protein fluorescence lifetime.

Conclusions:

  • The enhanced microscope system offers significant improvements in spatiotemporal resolution for SPT.
  • Correlative tracking and fluorescence lifetime measurements provide richer biological insights.
  • This advanced microscopy approach is expected to facilitate new correlative imaging and tracking studies.