Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-assembled chamber-like cardiac organoids for modeling cardiac chamber formation and cardiotoxicity assessment.

Nature communications·2026
Same author

Developmental chronology of mouse embryo from 2-cell stage through birth.

Nature cell biology·2026
Same author

In vitro mimicking of humanized cardiogenesis under porcine condition.

Cell & bioscience·2026
Same author

Cryo-EM structure of TMEM164 reveals distinct phospholipid remodeling mechanisms with anti-ferroptotic potential.

Nature communications·2025
Same author

Structure-guided screening identifies Tucatinib as dual inhibitor for MCT1/2.

EMBO reports·2025
Same author

Role of frustrations in cell reprogramming.

PNAS nexus·2025
Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026
Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026
Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026
Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026
Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026
Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jun 19, 2025

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.2K

Engineering mouse cell fate controller by rational design.

Tao Huang1,2, Dong Liu2, Xiaomin Wang2

  • 1College of Life Sciences, Zhejiang University, Hangzhou, China.

Nature Communications
|July 23, 2024
PubMed
Summary
This summary is machine-generated.

Engineered NanogBiD efficiently reprograms mouse cells by recruiting the Brg/Brahma-associated factor (BAF) complex. This tool enhances cell fate transition and chromatin accessibility for pluripotency.

More Related Videos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

8.8K
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

10.9K

Related Experiment Videos

Last Updated: Jun 19, 2025

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.2K
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

8.8K
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

10.9K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell fate decisions are governed by complex molecular machinery.
  • Identifying key components regulating these processes is crucial for understanding development and disease.

Purpose of the Study:

  • To design and test an engineered cell fate controller, NanogBiD, for enhanced somatic cell reprogramming.
  • To elucidate the molecular mechanisms by which NanogBiD facilitates cell fate transition.

Main Methods:

  • Fusion of the BRG1-interacting domain (BiD) of SS18 with Nanog to create NanogBiD.
  • Testing NanogBiD efficiency in mouse somatic cell reprogramming under various conditions.
  • Mechanistic studies involving chromatin accessibility assays and gene expression analysis.

Main Results:

  • Engineered NanogBiD demonstrated significantly higher efficiency in mouse somatic cell reprogramming compared to native Nanog.
  • NanogBiD facilitates cell fate transition by recruiting the Brg/Brahma-associated factor (BAF) complex.
  • This recruitment modulates chromatin accessibility and reorganizes cell-state-specific enhancers, leading to precocious activation of pluripotency genes.

Conclusions:

  • Engineered chromatin regulators like NanogBiD offer powerful tools for precise cell fate engineering.
  • The findings suggest potential therapeutic applications in the emerging field of cell-based therapies.
  • Further studies are needed to evaluate this approach in other species.