Analysis of N6-Methyladenosine Modification of HBV RNA by Methylated RNA Immunoprecipitation

Of all the chemical modifications of RNAs, the N6-methyladenosine (m⁶A) modification is the most prevalent and well-characterized RNA modification that is functionally implicated in a wide range of biological processes. The m⁶A modification occurs in hepatitis B virus (HBV) RNAs and this modification regulates the HBV life cycle in several ways. Thus, understanding the mechanisms underlying m6A modification of HBV RNAs is crucial in understanding HBV infectious process and associated pathogenesis. Here, we describe the currently utilized method in the detection and characterization of m6A-methylated RNAs during viral infection.