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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jun 19, 2025

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

pH-induced changes in IgE molecules measured by atomic force microscopy.

Jing Hu^1,2,3,4, Zuobin Wang^2,3,4,5, Dayong Jiang¹

¹School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun, China.

Microscopy Research and Technique

|July 24, 2024

Summary

This summary is machine-generated.

Protein structure is sensitive to its environment. This study reveals how pH affects Immunoglobulin E (IgE) molecule shape, size, and aggregation, offering insights into protein behavior under varying conditions.

Keywords:

IgE molecules imaging detection pH value ultrastructure

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Last Updated: Jun 19, 2025

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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Published on: March 6, 2017

Area of Science:

Biochemistry
Biophysics
Protein Science

Background:

Protein conformation and dynamics are influenced by their surrounding environment.
Understanding protein behavior under different conditions is crucial for biological and medical research.

Purpose of the Study:

To investigate the impact of pH on the ultrastructure of Immunoglobulin E (IgE) molecules.
To explore the relationship between pH and IgE morphology, height, and area.

Main Methods:

Utilized Atomic Force Microscopy (AFM) to image IgE molecules.
Incubated IgE molecules at various pH conditions to observe structural changes.

Main Results:

IgE molecule morphology, height, and area are highly sensitive to pH.
Acidic pH induced small-sized ellipsoidal IgE structures.
Alkaline pH promoted self-assembly into large, flower-like IgE aggregates.

Conclusions:

IgE molecule structure and aggregation are significantly pH-dependent.
The study provides a foundation for further investigations into IgE behavior under diverse physical and chemical factors.
The AFM methodology can be adapted to study IgE modifications induced by other environmental variables.