Exploring the Heat-Responsive miRNAs and their Target Gene Regulation in Ruditapes philippinarum Under Acute Heat...

Area of Science:

• Marine Biology
• Molecular Biology
• Aquaculture Science

Background:

• The Manila clam (Ruditapes philippinarum) aquaculture faces challenges from rising environmental temperatures.
• Understanding molecular responses to heat stress is crucial for industry sustainability.

Purpose of the Study:

• To investigate the molecular regulatory mechanisms of Ruditapes philippinarum under high-temperature stress.
• To identify key microRNAs (miRNAs) and their target genes involved in heat response.
• To enhance the sustainable development of the R. philippinarum aquaculture industry.

Main Methods:

• Differential expression profiling of miRNAs under acute heat stress.
• Identification of known and novel miRNAs.
• Bioinformatic analysis, including KEGG enrichment and miRNA-mRNA network construction.

Main Results:

• 46 known and 80 novel miRNAs were identified, with three significantly differentially expressed miRNAs (DEMs).
• KEGG analysis revealed 29 enriched pathways, highlighting roles for fatty acid metabolism, FoxO signaling, TGF-β signaling, and ubiquitin-mediated proteolysis.
• A regulatory map and miRNA-mRNA network were established.

Conclusions:

• This study elucidates critical molecular mechanisms governing Ruditapes philippinarum's response to high temperatures.
• The findings provide valuable insights for improving heat tolerance and sustainable aquaculture practices.