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Non-coding RNA alterations in occlusal disharmony-induced anxiety-like behaviour
Mi Zhang1, Ling Wu2,3, Sihui Zhang2
1Department of Oral Maxillofacial-Head Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Journal of Oral Rehabilitation
|July 25, 2024
Summary
Occlusal disharmony in mice induced anxiety-like behaviors and altered non-coding RNA (ncRNA) expression. CircRNA10039 and PTBP1 may be involved in this process.
Area of Science:
- Neuroscience
- Genetics
- Molecular Biology
Background:
- Occlusal disharmony (OD) is linked to anxiety-like behaviors, but the underlying molecular mechanisms are not fully understood.
- Non-coding RNAs (ncRNAs) are increasingly recognized for their roles in complex behaviors and neurological conditions.
Purpose of the Study:
- To investigate the expression profiles of ncRNAs in anxiety-like behavior induced by OD.
- To explore the biological functions and regulatory networks of dysregulated ncRNAs in this context.
Main Methods:
- Anxiety-like behavior was induced in C57BL/6 mice using anterior crossbite.
- Behavioral tests, corticosterone (CORT), and serotonin (5-HT) levels were measured.
- RNA sequencing identified differentially expressed (DE) ncRNAs, with RNA-binding protein interactions predicted via ENCORI and confirmed by western blot.
Main Results:
- OD successfully induced anxiety-like behaviors, evidenced by behavioral tests and elevated CORT levels.
- Significant dysregulation of ncRNAs was observed in OD mice.
- DE ncRNAs were enriched in anxiety-related pathways (GO and KEGG analyses). CircRNA10039 was upregulated and predicted to interact with PTBP1, which is associated with mRNA biogenesis and spliceosomes.
Conclusions:
- Anterior crossbite-induced OD leads to anxiety-like behaviors and concurrent changes in ncRNA expression.
- CircRNA10039 and PTBP1 are implicated as potential key players in OD-induced anxiety-like behaviors.
