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Updated: Jun 19, 2025

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Published on: May 21, 2015
Splenocytes and thymocytes migration patterns between lymphoid organs in pregnancy
Gabriela T Cruz-Cureño1, Marina Ch Rosales-Tarteaut1, Lourdes A Arriaga-Pizano2
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación Carpio s/n esq, Plan de Ayala, Plutarco Elías Calles, CP 11340, Ciudad de México, Mexico.
Background:
Cell migration is essential for the immune system and is frequently analyzed in adult non-pregnant animals but poorly explored in pregnant animals. However, a physiologic increased size in the spleen and periaortic lymph nodes had been reported in pregnant mice.
Methods:
Using a mouse model, we transferred PKH26-stained thymocytes and splenocytes from pregnant or non-pregnant animals to receptor mice in the presence or absence of pregnancy. Percentage of PKH-26 cells and Mean Fluorescence Intensity were calculated. Non-parametric ANOVA analysis was performed.
Results:
We detected that the percentage of PKH26+ thymocytes in the spleen, lymph nodes, and peripheral blood is higher in females than in males (p = 0.039). Our results showed a similar frequency of thymocytes and splenocytes from pregnant and non-pregnant mice located in receptor lymphoid organs (p > 0.05). Also, the location of marked cells was similar during the perinatal period (p > 0.05).
Conclusions:
The mobility of thymocytes and splenocytes in pregnant and non-pregnant mice is similar. Therefore, we suggest that the larger size of the spleen and periaortic lymph nodes noted previously in pregnant mice could result from the retention of leukocytes in the secondary lymphoid organs.
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