Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic Characterisation of Antimicrobial Resistance and Virulence of Animal-Derived <i>Klebsiella pneumoniae</i> Isolates from Germany, and Description of a Hypervirulent Strain.

Antibiotics (Basel, Switzerland)·2026
Same author

<i>Listeria ivanovii</i>-an underestimated pathogen in veterinary medicine.

Frontiers in veterinary science·2026
Same author

Clostridioides difficile in chicken fresh meat at retail in France.

Anaerobe·2026
Same author

A Single Amino Acid Substitution in CspA Increases Germination Sensitivity and Broadens Bile Salt Germinant Specificity in Clostridioides difficile Spores.

Environmental microbiology·2026
Same author

Comparison of Phenotypic and Genotypic Antimicrobial Resistances in Bovine <i>Clostridioides difficile</i> Isolates.

Antibiotics (Basel, Switzerland)·2026
Same author

Antimicrobial resistance, genetic diversity and virulence associated factors of <i>Campylobacter</i> spp. isolated from poultry meat in Algeria.

Frontiers in veterinary science·2026

Related Experiment Video

Updated: Jun 19, 2025

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

12.5K

Genomic study of European Clostridioides difficile ribotype 002/sequence type 8.

Ines Dost1,2, Mostafa Abdel-Glil1, Søren Persson3

  • 1Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Naumburger Straße 96a, 07743 Jena, Germany.

Microbial Genomics
|July 25, 2024
PubMed
Summary

Clostridioides difficile ST8/RT002 is a stable, adaptable lineage found across human and animal health sectors. Despite low genetic variation, it acquires antimicrobial resistance genes, highlighting One Health surveillance needs.

Keywords:
Clostridioides difficileOne HealthRT002ST8genomespangenomephylogenetic analysiszoonotic pathogen

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.2K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

8.6K

Related Experiment Videos

Last Updated: Jun 19, 2025

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
06:51

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

12.5K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.2K
Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
09:43

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP

Published on: October 30, 2016

8.6K

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Clostridioides difficile is a major cause of healthcare-associated infections.
  • Clostridioides difficile ribotype 002, linked to sequence type 8 (ST8), is a prevalent human pathogen.

Purpose of the Study:

  • To investigate the genetic characteristics of 537 Clostridioides difficile ST8/RT002 genomes.
  • To analyze antimicrobial resistance genes and mobile elements within this lineage.
  • To explore the One Health implications of ST8/RT002 epidemiology.

Main Methods:

  • Whole-genome sequencing of 298 C. difficile strains from a European collection.
  • Bioinformatic analysis integrating a global dataset of 1,437 ST8 genomes from Enterobase.
  • Pangenome analysis and core genome multilocus sequence typing (cgMLST) clustering.

Main Results:

  • ST8/RT002 genomes exhibit close genetic relatedness and low overall variation.
  • A diverse range of antimicrobial resistance genes and mobile elements were identified.
  • Clonal isolates were detected across human, animal, environmental, and food sectors, indicating a stable lineage with a potential universal environmental source.
  • Nine cross-sectoral cgMLST clusters were identified, despite a low percentage of non-human isolates.

Conclusions:

  • Clostridioides difficile ST8 is a prominent, genetically stable lineage with significant One Health implications.
  • The adaptability of ST8 in acquiring antimicrobial resistance genes underscores the need for vigilance.
  • Continued surveillance of non-human sources is crucial for a comprehensive understanding of C. difficile epidemiology.