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Published on: September 11, 2015
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Initial bone tissue reactions of hydroxyapatite/collagen-(3-glycidoxypropyl)trimethoxysilane injectable bone paste
Taira Sato1,2, Yuki Shirosaki3, Sho Oshima1,4
1Bioceramics Group, Biomaterials Field, Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Summary
This study shows that a novel hydroxyapatite/collagen bone paste with GPTMS is safe for bone repair. The paste is resorbed by osteoclasts without causing inflammation, promoting new bone formation.
Area of Science:
- Biomaterials Science
- Orthopedic Research
- Tissue Engineering
Background:
- A novel bioresorbable bone paste (HAp/Col-GPTMS) was previously developed.
- The paste demonstrated successful bone regeneration in porcine tibia defects within 3 months.
Purpose of the Study:
- To investigate the initial kinetics and bone tissue response of HAp/Col-GPTMS paste in rat tibiae.
- To assess the in vivo safety and resorption mechanisms of the bone paste.
Main Methods:
- Implantation of HAp/Col-GPTMS paste into rat tibial defects (directly injected and prehardened).
- Energy-dispersive X-ray spectrometry to detect GPTMS deposition.
- X-ray microcomputed tomography (μ-CT) for material resorption rates.
- Histological analysis for inflammatory response and osteoclast activity (TRAP staining).
Main Results:
- No GPTMS deposition detected in surrounding tissues at 1 day postimplantation.
- No abnormal inflammatory responses observed for either paste type.
- Material resorption rates decreased with increasing GPTMS concentration.
- Tartrate-resistant acid phosphatase (TRAP) positive cells (osteoclasts) were found at the paste periphery, indicating osteoclastic resorption.
- A correlation was found between TRAP activity and material resorption rate.
Conclusions:
- The eluted GPTMS from the HAp/Col-GPTMS bone paste is safe in vivo and does not cause adverse effects.
- Osteoclasts resorb the HAp/Col-GPTMS paste, similar to HAp/Col, facilitating bone remodeling.
- TRAP activity can be used to estimate the osteoclastic resorption ratio of bone regenerative materials.
