Related Experiment Video
Updated: Jun 19, 2025
06:13
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
Published on: December 9, 2022
3.9K
A Multiplexed Single-Cell Proteomic Workflow Applicable to Drug Treatment Studies
Colten D Eberhard1, Benjamin C Orsburn2
1The Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 25, 2024
Summary
Single-cell proteomics enables the analysis of heterogeneous cell responses to drugs. This study details methods for using highly multiplexed single-cell proteomics to identify drug-adapting cell subpopulations.
Area of Science:
- Proteomics
- Cell Biology
- Pharmacology
Background:
- Cellular responses to drug treatment are known to be heterogeneous.
- Quantitative analysis of single human cell proteomes is now possible via mass spectrometry.
Purpose of the Study:
- To detail current methods for applying highly multiplexed single-cell proteomics.
- To identify subpopulations of cells with unique drug responses and adaptation mechanisms.
Main Methods:
- Utilizing advances in mass spectrometry for quantitative single-cell proteomics.
- Applying highly multiplexed single-cell proteomics techniques to drug treatment studies.
Main Results:
- Emerging single-cell proteomics technology allows for rapid identification of cellular subpopulations.
- Methods are presented for analyzing drug response heterogeneity at the single-cell level.
Conclusions:
- Single-cell proteomics is a powerful tool for understanding drug response heterogeneity.
- The presented methods facilitate the identification of specific cellular adaptations to drug treatment.
