Discrepancies in fluorescent antibody, counterimmunoelectrophoresis, and Neufeld test for typing of Streptococcus...

The techniques of fluorescent antibody (FA) and counterimmunoelectrophoresis (CIE) were compared with the Neufeld test (quellung reaction) for typing of Streptococcus pneumoniae. A total of 88 isolates were examined by these three methods. Pool-, type-, or group-specific pneumococcal antisera were used in all three methods. Each isolate was initially tested with polyvalent antisera and all of the nine pools of antisera. Selection of the type- or group-specific antisera depended upon the reaction of the isolate with the pool sera. Sixty-eight of 88 (77.3%) isolates were positive using pool or typing sera and were correctly typed by CIE, while FA was found to be accurate for only 61 of 88 (69.3%) isolates. Positive reactions with more than one pool- or type-specific antisera, or no reaction, were seen with several of the isolates with both techniques. Even though CIE and FA are rapid and simple techniques, microbiologists should be cautious when utilizing them for typing of S. pneumoniae because of the discrepancies observed in this study.