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Related Experiment Video

Updated: Jun 19, 2025

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

Published on: January 26, 2019

RSV Neutralizing Antibodies in Dried Blood.

Jonne Terstappen^1,2, Eveline M Delemarre², Anouk Versnel²

¹Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, University Medical Center Utrecht, the Netherlands.

The Journal of Infectious Diseases

|July 25, 2024

Summary

Dried blood spot testing accurately measures respiratory syncytial virus (RSV) neutralization, offering a simpler alternative to traditional blood draws for vaccine and antibody trials. This patient-centered approach simplifies sample collection and processing.

Keywords:

antibodies clinical trials dried blood respiratory syncytial virus vaccine

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An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

Area of Science:

Virology
Immunology
Clinical Trials

Background:

Respiratory syncytial virus (RSV) neutralization is key for vaccine and monoclonal antibody (mAb) efficacy.
Traditional serology requires venipuncture, complicating sample acquisition, processing, storage, and transport in clinical trials.
Dried blood spot (DBS) sampling offers a simplified, cost-effective alternative.

Purpose of the Study:

To validate an assay for measuring RSV neutralization using dried capillary blood.
To assess the feasibility of DBS sampling as a patient-centered solution in clinical trials.

Main Methods:

Comparison of functional antibodies in matched serum and DBS samples from a Phase 1 trial of an anti-RSV prefusion F mAb (RSM01).
Quantification of half-maximal inhibitory concentration (IC50) using RSV-A2-mKate and Hep-2 cells.
Evaluation of DBS sample stability over time and under temperature stress.

Main Results:

High correlation (R2 = 0.98, P < .0001) between functional antibodies in DBS and serum.
Assay precision for DBS was comparable to serum.
mAb function remained stable in DBS for 9 months at room temperature and when frozen.

Conclusions:

Feasibility demonstrated for measuring RSV neutralization via DBS.
DBS testing presents a patient-centered alternative to serology for RSV and potentially other viral infections (influenza, SARS-CoV-2).
This method can streamline clinical trials and reduce associated costs.