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The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
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A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
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Updated: Jun 19, 2025

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Oppositely-charged silver nanoparticles enable selective SERS molecular enhancement through electrostatic

Yuqing Gu1, Siyi Wu1, Zhewen Luo1

  • 1State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 25, 2024
PubMed
Summary

Using oppositely charged silver nanoparticles enhances surface-enhanced Raman spectroscopy (SERS) for comprehensive molecular profiling of biological samples by overcoming analyte selectivity issues.

Keywords:
Charge reversalElectrostatic interactionOppositely-charged silver nanoparticlesSERS selective detectionSurface-enhanced Raman spectroscopy (SERS)

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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Biochemistry

Background:

  • Label-free surface-enhanced Raman spectroscopy (SERS) is a promising technique for molecular phenotyping.
  • SERS selectivity, driven by substrate-analyte interactions like electrostatic forces, can limit comprehensive molecular profiling.
  • Using only one type of charged substrate (negatively or positively) results in loss of information from certain analytes.

Purpose of the Study:

  • To investigate the use of both negatively- and positively-charged silver nanoparticles (Ag NPs) as SERS substrates.
  • To demonstrate how substrate charge influences SERS detection of charged molecules.
  • To explore strategies for acquiring more comprehensive SERS data from complex biological samples.

Main Methods:

  • Preparation of negatively-charged citrate-stabilized Ag NPs and positively-charged Ag NPs using a cetyltrimethyl-ammonium chloride-based protocol.
  • Utilizing these oppositely-charged Ag NPs as SERS substrates for detecting various charged molecules.
  • Analysis of SERS results and confirmation with molecular electrostatic potential calculations.

Main Results:

  • Both negatively- and positively-charged Ag NPs exhibited good dispersion and uniformity.
  • SERS detection clearly showed electrostatically-driven selective enhancement based on substrate charge.
  • Molecular electrostatic potential calculations supported the observed SERS selectivity.

Conclusions:

  • Surface charge modification of SERS substrates is crucial for optimizing analyte detection.
  • Employing combinations of oppositely-charged SERS substrates can significantly enhance the acquisition of molecular information from complex biological samples.
  • This approach offers a pathway to more comprehensive SERS sensing.