Fibrin aggravates periodontitis through inducing NETs formation from mitochondrial DNA

Related Experiment Video

Updated: Jun 19, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.3K

Yinan Chen1,2,3, Enhua Mei1,2,3, Shunxue Nan1,2,3

  • 1Department of Preventive Dentistry, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.

Oral Diseases
|July 26, 2024
PubMed
Abstract

Insights

Fibrin deposition in periodontitis promotes neutrophil extracellular trap (NET) formation. Mitochondria-derived reactive oxygen species (mtROS) drive this process, with a scavenger mitigating NETs and inflammation.

Area of Science:

  • Periodontology
  • Immunology
  • Cell Biology

Background:

  • Periodontitis involves inflammation and bone loss.
  • Neutrophil extracellular traps (NETs) contribute to inflammatory diseases.
  • Mitochondria play a role in cellular inflammation.

Purpose of the Study:

  • Investigate fibrin's role in NET formation during periodontitis.
  • Elucidate the involvement of mitochondria in NET formation.
  • Assess the therapeutic potential of targeting mitochondrial pathways.

Main Methods:

  • Utilized plasminogen-deficient (Plg-/-) mice to study fibrin deposition effects.
  • Conducted in vitro experiments on neutrophils exposed to fibrin.
  • Quantified mitochondrial reactive oxygen species (mtROS) using flow cytometry and immunofluorescence.
  • Evaluated the efficacy of mtROS scavenger Mito-TEMPO in vitro and in vivo.

Main Results:

  • Plg deficiency led to increased fibrin, neutrophil infiltration, inflammation, and bone resorption.
  • In vitro, fibrin induced inflammatory factors, mtROS, and NET formation (mtDNA-enriched).
  • Mito-TEMPO reversed these effects in vitro and reduced inflammation in Plg-/- mice.

Conclusions:

  • Fibrin deposition in gingiva induces NET formation in periodontitis.
  • Mitochondrial-derived mtROS are crucial mediators of fibrin-induced NET formation.
  • Targeting mtROS with Mito-TEMPO offers a potential therapeutic strategy for periodontitis.
Keywords:
fibrinmitochondrianeutrophilsneutrophils extracellular traps (NETs)periodontitisplasminogen

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