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Related Experiment Video

Updated: Jun 19, 2025

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
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Spatial Resolution for X-ray Excited Luminescence Chemical Imaging (XELCI).

Apeksha C Rajamanthrilage1, Unaiza Uzair1, Paul W Millhouse1

  • 1Department of Chemistry, Clemson University, Clemson, South Carolina 29634, United States.

Chemical & Biomedical Imaging
|July 26, 2024
PubMed
Summary
This summary is machine-generated.

X-ray excited luminescent chemical imaging (XELCI) enables chemical measurements through tissue. This technique overcomes limitations of traditional dyes, offering submillimeter spatial resolution for medical implant analysis.

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Area of Science:

  • Biomedical Engineering
  • Medical Imaging
  • Chemical Sensing

Background:

  • Measuring chemical concentrations on medical implants is crucial for understanding the local biochemical environment, particularly during infections.
  • Traditional chemical indicator dyes are limited in vivo due to background interference and light scattering through tissue.
  • X-ray excited luminescent chemical imaging (XELCI) offers a novel solution by using X-rays to excite luminescence on implant surfaces, minimizing scattering and background noise.

Purpose of the Study:

  • To evaluate the spatial resolution of X-ray excited luminescent chemical imaging (XELCI) through varying thicknesses of tissue.
  • To demonstrate the feasibility of XELCI for submillimeter chemical imaging on medical implants in a biologically relevant context.

Main Methods:

  • Developed a spectrochemical indicator film containing pH-indicator dyes placed over a scintillator layer on medical implants.
  • Utilized a focused X-ray beam to scan point-by-point, exciting luminescence and measuring absorption through tissue.
  • Assessed spatial resolution by imaging optically absorptive targets at different tissue depths (5 mm and 10 mm) using the knife-edge method.

Main Results:

  • Achieved a 20-80% knife-edge resolution of approximately 285 μm without tissue.
  • Measured spatial resolutions of 475 μm through 5 mm of tissue and 520 μm through 10 mm of tissue.
  • Demonstrated that increasing tissue depth had minimal impact on the recorded spatial resolution.

Conclusions:

  • XELCI provides high spatial resolution and implant surface specificity for chemical imaging through tissue.
  • The technique overcomes the limitations of conventional methods, showing significant promise for in vivo chemical sensing applications.
  • XELCI enables submillimeter chemical imaging through tissue, advancing the study of local biochemical environments around medical implants.