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Sorting differentiated mammalian cells using deterministic lateral displacement microfluidic devices.

Koji Matsuura1, Shingi Hashioka2, Koji Takata3

  • 1Department of Biosciences, Faculty of Life Science, Okayama University of Science, Okayama, Japan. kmatsuura@ous.ac.jp.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
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Summary

This study presents a label-free cell separation method using deterministic lateral displacement (DLD) microfluidic devices. The system effectively sorts differentiated cells based on size, advancing cell analysis and in vitro tissue models.

Keywords:
AdipocytesCell sortingDeterministic lateral displacementDifferentiated cellsMyocytes

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Label-free separation of differentiated and undifferentiated cells is crucial for in vitro cell analysis and clinical applications.
  • Current methods often require cell labeling, limiting their utility.

Purpose of the Study:

  • To develop and apply a deterministic lateral displacement (DLD) microfluidic system for label-free cell separation.
  • To sort differentiated cells based on size differences for improved cell analysis and tissue model preparation.

Main Methods:

  • A deterministic lateral displacement (DLD) microfluidic device was developed for passive cell separation.
  • Compressed air was used to drive fluid flow and control priming and sorting.
  • The system was tested on C2C12 mononuclear myocytes and multinuclear myotubes, and differentiated adipocytes from mouse embryonic fibroblast (MEF) cells.

Main Results:

  • Multinuclear myotubes were effectively sorted as larger particles using a DLD microfluidic channel with a critical diameter (Dc) of 20 μm.
  • Differentiated adipocytes containing lipid droplets, with diameters exceeding 20 μm, were successfully sorted.
  • Cell size was identified as a reliable parameter for differentiating cell types.

Conclusions:

  • The developed DLD microfluidic system enables label-free cell sorting based on size.
  • This technology supports advancements in single-cell analyses and the preparation of in vitro tissue models for drug discovery.