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Induction of Hypoxia in Living Frog and Zebrafish Embryos
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Hypoxia-Inducible Factor 1α Affects Yak Oocyte Maturation and Early Embryonic Development by Regulating Autophagy
Xin Ma1, Meng Wang1,2, Jinglei Wang1
1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Antioxidants (Basel, Switzerland)
|July 27, 2024
Summary
Hypoxia-inducible factor 1α (HIF-1α) enhances yak oocyte maturation and embryonic development by regulating autophagy. This study explored HIF-1α
Area of Science:
- Animal reproductive biology
- Cellular and molecular mechanisms
- Assisted reproductive technologies
Background:
- High-quality oocytes are essential for assisted reproductive technologies in animals.
- Yak reproductive cells are influenced by hypoxia-inducible factor 1α (HIF-1α) due to their adaptation to the Qinghai-Tibet Plateau.
- Autophagy plays a role in oocyte quality and development.
Purpose of the Study:
- To investigate the impact of HIF-1α on yak oocyte maturation and early embryonic development in vitro.
- To elucidate the role of autophagy in mediating HIF-1α's effects on yak oocytes.
- To explore potential strategies for improving yak assisted reproduction.
Main Methods:
- In vitro maturation of yak oocytes.
- Treatment with HIF-1α inducer (DFOM) and inhibitor (LW6).
- Assessment of oocyte maturation, early embryonic development, autophagy markers, CYP450s enzyme expression, and cumulus expansion factors.
- Inhibition of autophagy using 3-MA during DFOM treatment.
Main Results:
- DFOM upregulated HIF-1α, increasing cumulus expansion, first polar body expulsion rate, mitochondrial and actin levels, while decreasing ROS and apoptosis.
- DFOM promoted autophagy-related proteins, CYP450s enzymes, and cumulus expansion factors, enhancing oocyte maturation and embryonic development.
- LW6 showed opposite effects; inhibiting autophagy with 3-MA during DFOM treatment mimicked LW6's negative impacts on development and increased apoptosis.
Conclusions:
- HIF-1α regulates yak oocyte maturation and early embryonic development in vitro by modulating autophagy.
- HIF-1α influences cumulus expansion, oocyte quality (polar body expulsion, mitochondrial/actin levels, ROS, apoptosis), CYP450s, and expansion factors.
- These findings provide insights into yak reproductive mechanisms in hypoxia and suggest avenues for improving yak ART.
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