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Analysis of In Situ Electroporation Utilizing Induced Electric Field at a Wireless Janus Microelectrode
Haizhen Sun1,2, Linkai Yu1,2, Yifan Chen1,2
1School of Mechanical and Electric Engineering, Soochow University, Suzhou 215299, China.
This study introduces Janus particle microelectrodes for precise, in situ electroporation, enabling targeted intracellular delivery within microfluidic devices. The technique offers spatial control for cellular manipulation and therapeutic applications.
Area of Science:
- Biotechnology
- Cellular Engineering
- Microfluidics
Background:
- In situ electroporation enhances cell membrane permeability for intracellular delivery.
- Current methods face challenges in precise spatial control and targeting within native tissues.
Purpose of the Study:
- To develop an innovative Janus particle (JP)-based microelectrode for localized and controllable in situ electroporation.
- To investigate the effects of various parameters on cell membrane permeabilization and electroporation distribution.
- To demonstrate selective spatial control over cellular delivery areas.
Main Methods:
- Engineered a microfluidic chip with an indium tin oxide (ITO)-sandwiched microchannel and suspended JP microelectrodes.
- Developed a simulation model to analyze electroporation dynamics in single and chain-assembled cells.
- Systematically investigated parameters like pulse voltage, duration, medium conductivity, and JP radius.
Main Results:
- JP microelectrodes generate a stronger localized electric field, significantly enhancing electroporation.
- Transmembrane voltage and nanopore distribution are controllable by adjusting JP position, enabling selective electroporation.
- Differences in electroporation distribution in chain cells provide insights for tissue-level applications.
Conclusions:
- JP-based microelectrodes offer a robust, selective, and spatially controlled method for in situ electroporation.
- This technique provides a powerful alternative for precise cellular manipulation and therapeutic agent delivery.
- Findings offer directives for targeted modulation of specific cell populations in tissues and complex cellular processes.
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