Related Experiment Video
Updated: Jun 18, 2025

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies
Published on: September 13, 2024
Chemical Reaction Models in Synthetic Promoter Design in Bacteria
1Free University of Bozen-Bolzano, Faculty of Engineering, Bozen-Bolzano, Italy.
Abstract:
We discuss the formalism of chemical reaction networks (CRNs) as a computer-aided design interface for using formal methods in engineering living technologies. We set out by reviewing formal methods within a broader view of synthetic biology. Based on published results, we illustrate, step by step, how mathematical and computational techniques on CRNs can be used to study the structural and dynamic properties of the designed systems. As a case study, we use an E. coli two-component system that relays the external inorganic phosphate concentration signal to genetic components. We show how CRN models can scan and explore phenotypic regimes of synthetic promoters with varying detection thresholds, thereby providing a means for fine-tuning the promoter strength to match the specification.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Bacterial RNA Polymerase
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
The Eukaryotic Promoter Region
Reporter Genes
Antibiotic Selection
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

