Related Experiment Video

Updated: Jun 18, 2025

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Xylem Water Distribution in Woody Plants Visualized with a Cryo-scanning Electron Microscope

Published on: June 20, 2019

Protocol for collecting xylem sap from drought-treated tomato plants using a pressure chamber

Marzieh Davoudi¹, Antonios Petridis¹

¹Food Science Department, Aarhus University, 8200 Aarhus N, Denmark.

|July 28, 2024

Abstract

None:

When plants are subjected to drought, it is impossible to obtain xylem sap for subsequent biochemical and molecular analysis using root pressure exudate, the conventional approach. Here, we present a protocol for collecting xylem sap from drought-treated tomato plants using a pressure chamber. We describe steps for how to prepare plants, apply drought, and use the pressure chamber to collect the xylem sap. Using this technique, one can obtain 500-700 microL of xylem sap in just 5-7 min. For complete details on the use and execution of this protocol, please refer to Alexou and Peuke.¹.

Keywords:

Environmental sciences Plant sciences Systems biology

More Related Videos

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

Author Spotlight: Advancing Stomatal Research with Automated Aperture Measurement

Published on: February 9, 2024

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

Published on: April 5, 2013

Related Concept Videos

Xylem and Transpiration-driven Transport of Resources

Xylem and Transpiration-driven Transport of Resources

The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Articles linked to this work by shared authors, journal, and citation graph.

Adaptive responses to elevated CO2 in fruit species with different phloem loading mechanisms.

Frontiers in plant science·2024

Fungicide Resistance in Botrytis spp. and Regional Strategies for Its Management in Northern European Strawberry Production.

Biotech (Basel (Switzerland))·2023

The mutation of C-24 reductase, a key enzyme involved in brassinolide biosynthesis, confers a novel compact plant architecture phenotype to cucumber.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2022

Reducing toxic reactive carbonyl species in e-cigarette emissions: testing a harm-reduction strategy based on dicarbonyl trapping.

RSC advances·2022

Genome-Wide Identification and Expression Analysis of Heat Shock Protein 70 (HSP70) Gene Family in Pumpkin (Cucurbita moschata) Rootstock under Drought Stress Suggested the Potential Role of these Chaperones in Stress Tolerance.

International journal of molecular sciences·2022

Long-distance control of the scion by the rootstock under drought stress as revealed by transcriptome sequencing and mobile mRNA identification.

Horticulture research·2022

See all related articles