Evaluation of environmental contamination in beeswax products

Beeswaxes are used as a coating agent or as a wrapping material for food products making them potentially ingested by consumers. There is no regulation yet in Europe giving maximum levels of contaminants in this type of product. Nevertheless, being a natural product, they are exposed to environmental pollution, thus it appears necessary to establish their contamination rate in order to evaluate potential human exposure. In this study, a method of extraction of different environmental contaminants including pesticides, phthalates, PAHs and phenols was developed. Based on a hot Soxhlet extraction, followed by cleaning steps, the method was validated for the quantitation of the cited contaminants by LC-MS/MS and GC-(MS)/MS. Three different types of waxes were analyzed including typical white waxes (Cera Alba) and yellow waxes (Cera Flava). It was shown that all waxes had the presence of at least one contaminant and that phthalates, in particular DEHP, was present in all beeswax samples. Insecticides were found in majority among all the classes of pesticides screened. The yellow waxes were found to be contaminated with the highest rates of PAHs (60%), pesticides (75%) and phenols (40%). The detection frequency of PAHs, in contrast to phthalates, was the lowest for all the types of waxes combined.