Related Experiment Video

Updated: Jun 18, 2025

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

14.9K

Translational control of papillomavirus mRNAs in the spotlight

Alejandra García1, Giovanna Maldonado1, Greco Hernández2

  • 1mRNA and Cancer Laboratory, Unit of Biomedical Research on Cancer, National Institute of Cancer (INCan), Mexico City 14080, Mexico.

Trends in Cell Biology
|July 28, 2024
PubMed

Insights

High-risk human papillomaviruses (HPVs) cause cervical cancer, with viral oncoprotein synthesis controlled by translation. Targeting protein synthesis may offer new therapies to improve patient survival.

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • High-risk human papillomaviruses (HPVs) are the primary cause of cervical cancer globally.
  • Cervical cancer incidence and mortality are rising worldwide.
  • Viral oncoprotein synthesis is significantly regulated by translational control mechanisms.

Purpose of the Study:

  • To explore the role of translational control in high-risk HPV infection.
  • To investigate novel therapeutic strategies targeting protein synthesis machinery for cervical cancer.

Main Methods:

  • Analysis of translational regulation of viral oncoproteins.
  • Investigation of protein synthesis pathways in HPV-infected cells.

Main Results:

  • Demonstrated strong translational control over the synthesis of viral oncoproteins.
  • Identified protein synthesis machinery as a key target in HPV-driven cancers.

Conclusions:

  • Targeting protein synthesis represents a promising therapeutic avenue for cervical cancer.
  • Interfering with translational control could enhance treatment efficacy and patient survival in cervical cancer.
Keywords:
cervical cancereIF4AeIF4Epapillomavirustranslational control

More Related Videos

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells09:20

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

15.7K
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

18.2K

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Initiation of Translation02:33

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
32.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
880
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.6K