Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The osteoinductive capacity of differently HCl-decalcified bone alloimplants.

C Delloye, A Hebrant, E Munting

    Acta Orthopaedica Scandinavica
    |August 1, 1985
    PubMed
    Summary

    Bone HCl-decalcification procedures enhance bone inductive implant yield. Preservation methods like freezing or freeze-drying did not significantly alter the osteoinductive capacity of these implants.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development and implementation of novel liquid biopsy NGS panels via the OncNGS precommercial procurement (PCP) initiative.

    ESMO open·2025
    Same author

    Time for a true integrated approach to spine care.

    Brain & spine·2023
    Same author

    Affordable spine care.

    Brain & spine·2022
    Same author

    PRECISION: the Belgian molecular profiling program of metastatic cancer for clinical decision and treatment assignment.

    ESMO open·2022
    Same author

    Spine Tango annual report 2012.

    European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2013
    Same author

    Hyperosmotic stress induces cell cycle arrest in retinal pigmented epithelial cells.

    Cell death & disease·2013

    Area of Science:

    • Biomaterials Science
    • Orthopedic Research
    • Tissue Engineering

    Background:

    • Bone inductive implants are crucial for bone regeneration.
    • Optimizing processing methods for bone grafts is essential for clinical success.
    • Understanding the impact of decalcification and preservation on osteoinductivity is key.

    Purpose of the Study:

    • To evaluate the osteoinductive capacity of bone implants processed via different hydrochloric acid (HCl) decalcification methods.
    • To compare the effects of deep-freezing and freeze-drying preservation techniques on bone implant osteoinductivity.
    • To determine the optimal procedure for obtaining bone inductive implants.

    Main Methods:

    • Heterotopic implantation of processed bone grafts in allogeneic rats.

    Related Experiment Videos

  • Three decalcification procedures: antigen-extracted autolysed implant (AAA bone), HCl-decalcification at 4°C, and HCl-decalcification at room temperature.
  • Evaluation of bone inductive capacity using isotopic strontium incorporation and ash-weight measurements at 2 months post-implantation.

    • Main Results:

    • HCl-decalcification alone, regardless of temperature (4°C or room temperature), resulted in a higher new bone yield compared to freeze-dried AAA bone.
    • Preservation techniques (deep-freezing vs. freeze-drying) did not significantly impact the osteoinductive capacity of the implants, with the exception of AAA bone.
    • The study identified HCl-decalcification as a superior method for enhancing bone inductive implant properties.

    Conclusions:

    • Hydrochloric acid decalcification is an effective method for enhancing the bone inductive capacity of implants.
    • Short-term preservation techniques have minimal impact on the osteoinductivity of HCl-decalcified bone implants.
    • These findings provide valuable insights for optimizing bone graft processing for orthopedic applications.