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Related Experiment Video

Updated: Jun 18, 2025

Postoperative Ileus Murine Model
04:26

Postoperative Ileus Murine Model

Published on: July 12, 2024

564

Postoperative Ileus Murine Model.

Dan Tian1, Bihan Liu1, Shiyang Huang2

  • 1General Surgery Department, Beijing Friendship Hospital, Capital Medical University; National Clinical Research Center for Digestive Diseases; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University; Beijing Laboratory of Oral Health, Capital Medical University School of Stomatology.

Journal of Visualized Experiments : Jove
|July 29, 2024
PubMed
Summary

Researchers developed a new mouse model for postoperative ileus (POI) using intestinal manipulation. This model helps study POI mechanisms and potential new treatments for this common surgical complication.

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Last Updated: Jun 18, 2025

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Area of Science:

  • Gastroenterology
  • Surgical Research
  • Animal Models

Background:

  • Postoperative ileus (POI) is a common complication following abdominal surgery, leading to increased patient morbidity and healthcare costs.
  • The exact mechanisms underlying POI, including neuronal, epithelial, and inflammatory factors, remain incompletely understood.
  • Effective experimental models are essential for investigating POI pathophysiology and developing targeted therapies.

Purpose of the Study:

  • To establish and validate a murine model of POI that mimics clinical surgical conditions.
  • To utilize this model for studying the mechanisms of intestinal dysfunction and damage after abdominal surgery.

Main Methods:

  • A murine model of POI was created using controlled intestinal manipulation (IM) via abdominal massage with a cotton swab.
  • Gastrointestinal transit was assessed 24 hours post-IM using FITC-dextran gavage and fluorescence imaging.
  • Histological analysis (hematoxylin and eosin staining) and flow cytometry were employed to evaluate submucosal swelling and immune cell infiltration.

Main Results:

  • Intestinal manipulation significantly delayed gastrointestinal transit in mice 24 hours after the procedure.
  • The model demonstrated characteristic POI features, including submucosal tissue swelling and increased immune cell infiltration.
  • The study identified optimal IM pressure and a hyperemic effect as critical procedural elements.

Conclusions:

  • The developed murine model of POI via intestinal manipulation accurately reflects clinical scenarios.
  • This model provides a valuable platform for investigating the molecular and cellular mechanisms of POI.
  • It can be used to test and develop novel therapeutic strategies for preventing or treating postoperative ileus.