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Simian retrovirus transmission in rhesus macaques

Peter Nham1, Bryson Halley1, Koen K A Van Rompay1,2

  • 1California National Primate Research Center, University of California, Davis, California, USA.

Journal of Medical Primatology
|July 29, 2024
PubMed

Insights

Generating Simian Retrovirus (SRV) positive controls using in vivo passage in rhesus macaques has recently become more difficult. This study investigates the reasons behind the decreased transmissibility of SRV-1 in these animals.

Area of Science:

  • Veterinary Virology
  • Primate Virology
  • Infectious Disease Research

Background:

  • Historically, in vivo passage in rhesus macaques was a reliable method for generating Simian Retrovirus (SRV) positive control materials.
  • Recent attempts to generate SRV positive controls using this established method have unexpectedly failed, indicating a significant change in transmissibility.

Purpose of the Study:

  • To investigate the reasons behind the recent difficulties in transmitting Simian Retrovirus (SRV) via in vivo passage.
  • To understand the factors contributing to the decreased success rate of generating SRV positive control materials in rhesus macaques.

Main Methods:

  • The study involved reviewing historical protocols for in vivo passage of SRV.
  • Observations from recent failed transmission attempts were analyzed.
  • Exploratory analysis was conducted to identify potential reasons for the diminished SRV transmissibility.

Main Results:

  • The traditional method of inoculating uninfected rhesus macaques with whole blood from SRV-1 infected macaques is no longer consistently successful.
  • There has been a notable decrease in the ability to transmit SRV through in vivo passage in the study population.
  • The study identified a shift in the ease of SRV transmission in this experimental model.

Conclusions:

  • The efficacy of in vivo passage for generating Simian Retrovirus (SRV) positive controls has significantly declined.
  • Further research is needed to elucidate the specific factors responsible for the reduced SRV transmissibility in rhesus macaques.
  • Understanding these changes is crucial for maintaining reliable SRV diagnostic and research materials.
Keywords:
infectionnon‐human primatesequencingtransfusion

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