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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Updated: Jun 15, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Fuel-Driven Dynamic Combinatorial Peptide Libraries.

Fabian Späth¹, Héctor Soria-Carrera¹, Michele Stasi¹

¹School of Natural Sciences, Department of Bioscience, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.

Angewandte Chemie (International Ed. in English)

|July 29, 2024

Summary

This summary is machine-generated.

This study introduces a new dynamic combinatorial chemistry (DCC) approach using peptide-based macrocycles that operate out-of-equilibrium. This method enables the engineering of dynamic libraries for tunable material systems with emergent properties.

Keywords:

Chemical reaction cycle Complex coacervation Dynamic combinatorial library Liquid-liquid phase separation Simple coacervation

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Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

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Published on: June 20, 2014

Area of Science:

Chemical Synthesis
Materials Science
Supramolecular Chemistry

Background:

Dynamic combinatorial chemistry (DCC) typically operates near equilibrium, limiting control over library member behavior.
Self-assembly in DCC can lead to selection and replication, but out-of-equilibrium systems offer greater kinetic and thermodynamic regulation.

Purpose of the Study:

To develop a novel DCC approach utilizing a catalytic reaction cycle to generate transient, out-of-equilibrium peptide-based macrocycles.
To explore the liquid-liquid phase separation behavior of these out-of-equilibrium macrocycle libraries.
To demonstrate the engineering of peptide structures for controlling dynamic library behavior.

Main Methods:

Utilized a catalytic reaction cycle to create a chemically fueled dynamic combinatorial library.
Synthesized peptide-based macrocycles from a diverse range of amino acids.
Investigated liquid-liquid phase separation properties of the generated molecular libraries.

Main Results:

Successfully generated transient, out-of-equilibrium peptide-based macrocycles.
Demonstrated that library members' behavior is regulated by kinetics and thermodynamics, not just equilibrium.
Showcased the ability to engineer peptide structures to control the dynamic library's properties, including phase separation.

Conclusions:

This work advances DCC by enabling out-of-equilibrium operation through catalytic cycles.
Engineered peptide macrocycles can control dynamic library behavior, paving the way for tunable materials.
The findings have implications for developing novel materials and understanding biological chemistry.