Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Batteries and Fuel Cells03:12

Batteries and Fuel Cells

A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
Chemiosmosis and ATP Synthesis01:22

Chemiosmosis and ATP Synthesis

The electron transport chain is a critical component of cellular respiration, occurring in the inner mitochondrial membrane. It facilitates the transfer of high-energy electrons from reduced cofactors NADH and FADH₂ to molecular oxygen, the final electron acceptor. This transfer of electrons through a series of protein complexes is tightly coupled to the translocation of protons across the membrane, generating a proton gradient essential for ATP synthesis.Electron Flow and Proton...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spinel-engineered cobalt oxide nanoflowers for dual-function electrocatalysis: Synergistic PET plastic upcycling and hydrogen production.

Journal of hazardous materials·2026
Same author

Reversible Nucleolar Complex Coacervation by Short Cationic Peptides.

Journal of the American Chemical Society·2026
Same author

Diagnostic sensitivity and safety of visceral pleural biopsy under semi-rigid medical thoracoscopy in patients with undiagnosed exudative pleural effusions: a retrospective study.

Therapeutic advances in respiratory disease·2026
Same author

Structural analysis of an extracellular polysaccharide of Lactobacillus plantarum ZHR8 and its role in preventing intestinal inflammation.

International journal of biological macromolecules·2026
Same author

Unveiling muscle fatigue: identifying key gene biomarkers and therapeutic targets.

Molecular and cellular biochemistry·2026
Same author

Enzyme-Free Phosphorylation with Kinetic Gating in a De Novo Coiled-Coil System.

Journal of the American Chemical Society·2026
Same journal

Application of Screen Printing in Perovskite Solar Devices.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

One-Shot Pd(II)-Catalyzed Multiple C-H Activation Enables Modular Construction of Fluorenylidene Oxindole-Based Multi(Polycyclic) Aromatic Enes.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Rapid Assembly of a Covalently Locked Organic Cage Revealing Symmetry-Matched Guest Recognition.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Expanding Cyanide-Bridged Weakly Coordinating Anions Through the Brominated Silver Salt Ag[BCNB<sup>Br</sup>].

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Photoexcited Nickel(0)-Catalyzed Direct Decarboxylative Cross-Coupling.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Divergent Total Syntheses of Bisnicalaterine Alkaloids Enabled by a Stereocontrolled Geissoschizol Synthesis.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures
08:02

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

Design of Fuel-Dependent, Complex-Coacervate-Based Synthetic Cells.

Anna-Lena Holtmannspötter1, Xiaoyao Chen1, Yiyi Fu1

  • 1Department of Bioscience, School of Natural Sciences, Technical University of Munich, Garching, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|May 7, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed new fuel-dependent synthetic cells using peptides. Incorporating tryptophan enhances droplet stability and waste resistance, enabling RNA compatibility and offspring production for synthetic life research.

Keywords:
coacervatesfuel‐dependent synthetic cellspeptide designpeptidessynthetic biology

More Related Videos

Cell-Free Protein Synthesis System for Building Synthetic Cells
07:43

Cell-Free Protein Synthesis System for Building Synthetic Cells

Published on: April 19, 2024

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
09:37

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Published on: April 27, 2020

Related Experiment Videos

Last Updated: May 8, 2026

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures
08:02

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

Cell-Free Protein Synthesis System for Building Synthetic Cells
07:43

Cell-Free Protein Synthesis System for Building Synthetic Cells

Published on: April 19, 2024

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
09:37

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Published on: April 27, 2020

Area of Science:

  • Synthetic Biology
  • Biochemistry
  • Materials Science

Background:

  • Fuel-dependent synthetic cells require fuel for emergence and sustenance, decaying under starvation.
  • Previous designs faced challenges with waste product accumulation and unknown lifetime parameters.
  • Droplet-based synthetic cells offer a promising route towards synthetic life.

Purpose of the Study:

  • To establish design rules for chemically fueled complex coacervate droplets using hexapeptides.
  • To investigate parameters influencing synthetic cell lifetime and division capabilities.
  • To overcome waste accumulation issues in fuel-dependent synthetic cell designs.

Main Methods:

  • Utilized hexapeptides with a focus on incorporating tryptophan for enhanced polyanion affinity.
  • Investigated the impact of tryptophan placement within the peptide sequence on droplet properties.
  • Employed RNA as a polyanion to assess offspring production and waste resistance.

Main Results:

  • Tryptophan incorporation significantly increased peptide affinity for polyanions, improving droplet formation and stability.
  • Synthetic cells demonstrated extended lifetimes and enhanced resistance to waste product accumulation.
  • RNA-compatible synthetic cells successfully produced offspring, indicating improved functionality.

Conclusions:

  • Design rules for tryptophan-containing hexapeptides yield more robust and functional fuel-dependent synthetic cells.
  • These advancements facilitate the creation of waste-resistant, RNA-compatible systems for synthetic life studies.
  • The findings pave the way for next-generation synthetic cells with improved longevity and reproductive capabilities.